Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTLKNAYIIDAIRTPFGRYAGGLASVRADDLGAVPIKALMQRNPNVDWEQVDDVIYGCANQAGEDNRNVGRMSALLAGLPYQVPATTINRLCGSSLDAIAIAARAIKAGEANLVIAGGVESMSRAPYVMGKSDSAFGR-SQKIEDTTMGWRFINPKLKELYGVDTMPQTAENVAEQFNVNRADQDQFALVSQQRTASAQAKGFFSKEIVAVEIPQRKGDAVVIDTDEHPRASTTLEGLSKLKPVVKADGTVTAGNASGINDGAAALLIASDDAVQAYNLKPRAKIIASTAVGVEPRIMGFAPAPAIKKLFKQANLTLDQMDVIELNEAFAAQALAVTRDLGLPDNSDKVNPNGGAIALGHPLGASGARLVTTALNQLEQTGGRYALCSMCIGVGQGIALIVERVEAL 4O9C Chain:E ((1-392)) --MTDVVIVSAARTAVGKFGGSLAKIPAPELGAVVIKAALERA-GVKPEQVSEVIMGQVLTAG-SGQNPARQAAIKAGLPAMVPAMTINKVSGSGLKAVMLAANAIMAGDAEIVVAGGQENMSAAPHVLPGSRDGFRMGDAKLVDTM-----IVDGLWDVYNQYHMGITAENVAKEYGITREAQDEFAVGSQNKAEAAQKAGKFDEEIVPVLIPQRKGDPVAFKTDEFVRQGATLDSMSGLKPAFDKAGTVTAANASGLNDGAAAVVVMSAAKAKELGLTPLATIKSYANAGVDPKVMGMGPVPASKRALSRAEWTPQDLDLMEINEAFAAQALAVHQQMGWDTS--KVNVNGGAIAIGHPIGASGCRILVTLLHEMKRRDAKKGLASLCIGGGMGVALAVER----

General information: TITO was launched using: RESULT: Template: 4O9C.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 2717 -96557 -35.54 -246.95 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain E : 0.83 3D Compatibility (PKB) : -35.54 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.505

(partial model without unconserved sides chains): PDB file : Tito_4O9C.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4O9C-query.scw PDB file : Tito_Scwrl_4O9C.pdb: