Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPRIKVLPHAQFCPEGAEFEVEQNANLCQSLLNKGIK-IEHACDMSCACTTCHVIVRKGF-DSLEEMNDVEADLLDRAWGLEPDSRLSCQVKVVDED---LEIEIPKYTINHASENH 3HUI Chain:A ((21-125)) MAKINFVDH---TGETRTVEVEEGATVMEAAIRNAIPGVEAECGGACACATCHVYVDEAWREKVGGPSPMEEDMLDFGYDVRPNSRLSCQIKVS-NELDGLIVTTPERQ--------

General information: TITO was launched using: RESULT: Template: 3HUI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 501 1154 2.30 11.54 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.82 3D Compatibility (PKB) : 2.30 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.496

(partial model without unconserved sides chains): PDB file : Tito_3HUI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3HUI-query.scw PDB file : Tito_Scwrl_3HUI.pdb: