Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKVQVKLLDPRLGKEWPLPSYATAGSAGLDLRACLDEAIEIEPGQTVLVKTGMAIYIHDVNFAGLILPRSGLGHKHGIVLGNLVGLIDSDYQGELMVSVWNRGQTT-FRLEPGERLAQYVLVPVVQAEFEQVEEFEETLRGAGGFGHTGKQ 1SM8 Chain:A ((29-152)) ----------RLDPGLPLPSRAHDGDAGVDLYSA--EDVELAPGRRALVRTGVAVAV-PFGMVGLVHPRSGLATRVGLSIVNSPGTIDAGYRGEIKVALINLDPAAPIVVHRGDRIAQLLVQRVELVELVEVSSFDE--------------

General information: TITO was launched using: RESULT: Template: 1SM8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 519 -59674 -114.98 -485.15 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -114.98 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.395

(partial model without unconserved sides chains): PDB file : Tito_1SM8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1SM8-query.scw PDB file : Tito_Scwrl_1SM8.pdb: