Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKTETTIQGLAASLNPARAARKIIGFVPTMGNLHEGHLTLVREAKKLCDVVVVSIFVNPTQFGPGEDFDNYPRTLEQDSRLLADVGCDIIFAPSVEQMYGTQPRLTNISVSQITDDLCGSSRPGHFDGVALVVTKLFNIVQPNYAFFGQKDYQQLAVIRQFVQDLNIPLEVIGVPIVRAEDGLALSSRNGYLTPEQRQVAPVIYQGLKQAEEQLHQGKD-LQQVLADLKTLLTDNG-FVVDYVEARQPNLLAASQ-FDRDIVLFVAAKLGGTRLIDNLQVAFTPQ 2EJC Chain:A ((1-280)) MRIIETIEEMKKFSEEMREKKKTIGFVPTMGYLHEGHLSLVRRARAENDVVVVSIFVNPTQFGPNEDYERYPRDFERDRKLLEKENVDCIFHPSVEEMYPP-DFSTYVEETKLSKHLCGRSRPGHFRGVCTVVTKLFNIVKPHRAYFGQKDAQQFRVLRRMVRDLNMDVEMIECPIVREPDGLAMSSRNVYLSPEERQQALSLYQSLKIAENLYLNGERDAEKIKEEMIKHLSRFDKVKIDYVEIVDEETLEPVEKIDRKVIVAVAAWVGNARLIDNTILG----

General information: TITO was launched using: RESULT: Template: 2EJC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1417 -136783 -96.53 -493.80 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.84 3D Compatibility (PKB) : -96.53 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.624

(partial model without unconserved sides chains): PDB file : Tito_2EJC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2EJC-query.scw PDB file : Tito_Scwrl_2EJC.pdb: