Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MILFGKKKVKPSQKAYAVVTGAGSGIGRSFAIELAKRGGSVVCADINLEAAEETVTLLEQQGAKAFAMHCDVGDPEHVTNLAETAEILLSHPVTLVINNAGVGLGGKFDELSLEDWNWVMNINLWGVIHGCHAFVPKFKKL-GYGAIINVASAASYTAAPEMTAYNVTKAGVRALSETLSAELRKFN--IKVNVLCPTLVPTNIIKNGRIPGRYS----KL---------ADHALMNYALTTSDDVAKLTLNRLDQDELYTIPQIDAKLFWLMKRASPSLYTKFLGTSYKLFK 5IG2 Chain:B ((9-263)) --------MFEFDGKVAVITGAGSGFGRAFAEKGASLGMKLVLADVDEGALARTVDTLRAAGAEVIGVRTDVSNGAQVQALADAALEAFGK-VHLLFNNAGVGAGGFLWESSANDWAWVFGVNVMGVAHGVRVFAPIMLGQNEAAHIVNTASVAGLLSPPSMGIYNASKHAVVSLTETLYHDLRNAGGEVGCSLLCPAFVPTGIADAERVRPEALRNEAQPTRSQLAADRQLQRAVRSGKLGATDVATLTFEAIAERRFYILTH-----------------------------

General information: TITO was launched using: RESULT: Template: 5IG2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1342 -93592 -69.74 -391.60 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain B : 0.75 3D Compatibility (PKB) : -69.74 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.572

(partial model without unconserved sides chains): PDB file : Tito_5IG2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5IG2-query.scw PDB file : Tito_Scwrl_5IG2.pdb: