Modeling by threading (Tito software)
Unconserved sides chains calculation (Scwrl software)
Evaluation (QMean software)



Input alignment information:
Query sequenceMFQHIPPYAGDPILSLMEQFNADTRSEKVNLSIGLYYNED-SIVPQLETIIEAQKRIEPKNGKTKLYLPMEGFKPYREAIQALLFGANS--PAVKAGRAVTIQTLGGSGALKVGADFLKTYFP--N-SDVWVSQPTWDNHVAIFNGAGIKTHFYPYFDAETRGVDFDGMLSTLKTL-PEQSIVLLHPCCHNPTGADLNPAQWDQVIAVLKDRNLIPFLDIAYQGFGDGMEEDAYAIRALDQAGLNFIVSNSFSKIFSLYGERVGGLTFVCDDAEAAQCTFGQLKATVRRIYSSPPTTGAWLVDEVLNDAELNQQWQGEVKEMRERIIKMRSILKDELTKALPDRDFSYLVNQKGMFSYTGLTAEQVDILREEYAIYLVRSGRICVAGLNMNNVYTVAKAMAEVLAKSVEAA
4F5L Chain:A ((12-406))-FENITAAPADPILGLADLFRADERPGKVDLGVGV-YKDETGKTPVMTSVKKAEQYLLE-NETTKTYLGLDGLPEFGRCTQELLFGKGSALINDKRART--AQTPGGSGALRVAADFLA---KNTSVKRVWVSNPTWPNHKAIFNSAGLEVREYAYYDAENHTLDFDALINSLNEAQAGDVVLFH-GCCHNPTGADPTLEQWQTLAQLSVEKGWLPLIDIAYQGFGRGLEEDAEGLRAFAAMHKELIVASSCSKNFGLYNERVGACTLVAADSETVDRAFSQMKAAIRANYSSPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGANRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRINVAGMTPDNMAPLCEAIVAVL-------


General information:
TITO was launched using:
RESULT:

Template: 4F5L.pdb
Alignment : align.pir
Tito was launched with SMD and SCWRL
Tito text output
Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2063 37533 18.19 96.73
target 2D structure prediction score : 0.71
Monomeric hydrophicity matching model chain A : 0.82

3D Compatibility (PKB) : 18.19
2D Compatibility (Sec. Struct. Predict.) : 0.71
1D Compatibility (Hydrophobicity) : 0.82
QMean score : 0.534

(partial model without unconserved sides chains):
PDB file : Tito_4F5L.pdb:





(Unconserved sides chains are recalculated) :
Sequence: align-4F5L-query.scw
PDB file : Tito_Scwrl_4F5L.pdb: