Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLPPETLEEELDYIYESNNNLLLLAIY-EGTIIGTASVKADSQFRLSHVGEV-GISILQEYWGMGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDLGEFLDVYKMSYLIE 2J8M Chain:B ((3-165)) ------ASIRDAGVADLPGILAIYNDAVGNTTA-IWNET--PVDLANRQAWFDARAR-QGYPILVASDAAGEVLGYASYGDWRPFEGFRGTVEHSVYVRDDQRGKGLGVQLLQALIERARAQ-GLHVMVAAIESGNAASIGLHRRLGFEISGQMPQVGQK-FGRWLDLTFMQLNL-

General information: TITO was launched using: RESULT: Template: 2J8M.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 723 -42545 -58.84 -264.25 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain B : 0.68 3D Compatibility (PKB) : -58.84 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.434

(partial model without unconserved sides chains): PDB file : Tito_2J8M.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2J8M-query.scw PDB file : Tito_Scwrl_2J8M.pdb: