Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTVTIDWENLGFSYMKLPYRYLAHFKNGQWDQGELTEDATLHISESSPSLHYGQQAFEGLKAYRTKDGSVQLFRPDENAKRLQRTCDRLLMPQVPTDMFVEACKAVVRANEEYVPLYGIGGTLYLRPLLIGVGDIIGVKPAEEYIFTIFAMPVGNYFKG-GLVPTNFLIQDEYDRAAPNGTGAAKVGGNYAASLLPGKMAKSRHFSDVIYL-DPSTHTKIEEVGSANFFGIT--ADN--EFVTP-LSPSILPSITKYSLLYLAEHRL-GLTPIEGDVPIDNLDRF------VEAGACGTAAVISPIGGIQHGDDFHVFYSETE-VGPVTRKLYNELTGIQFGDIEAPEGWIVKVD 1EKV Chain:B ((24-365)) -----PGEPLVFGKTFTDHMLMVEWNDKGWGQPRIQPFQNLTLHPASSSLHYSLQLFEGMKAFKGKDQQVRLFRPWLNMDRMLRSAMRLCLPSFDKLELLECIRRLIEVDKDWVPDA-AGTSLYVRPVLIGNEPSLGVSQPRRALLFVILCPVGAYFPGGSVTPVSLLADPAFIRAWVGGVGNYKLGGNYGPTVLVQQEALKRGCEQVLWLYGP--DHQLTEVGTMNIFVYWTHEDGVLELVTPPLNGVILPGVVRQSLLDMAQ-TWGEFRVVERTITMKQLLRALEEGRVREVFGSGTACQVCPVHRILYKDRNLHIPT-MENGPELILRFQKELKEIQYGIR--AHEWMFPV-

General information: TITO was launched using: RESULT: Template: 1EKV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1751 -130481 -74.52 -399.02 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain B : 0.75 3D Compatibility (PKB) : -74.52 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.430

(partial model without unconserved sides chains): PDB file : Tito_1EKV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1EKV-query.scw PDB file : Tito_Scwrl_1EKV.pdb: