Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNVGCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGLDSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFFELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQEAGIKTYAHHIAKEITKN-NDEYTVLFENGE-TLTGDCVLFAGGRVPNTEKLGLENTNVELDKKGFIKVDKYQNTTDEHIFAVGDVIGK----LDLTPVAIAAGRRLSERLFNGKKDSYLDYKLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALND--Y-RQ-KCEMKLICVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR 1ONF Chain:A ((2-495)) --VYDLIVIGGGSGGMAAARRAARHNAKVALVEKSRLGGTCVNVGCVPKKIMFNAASVHDIL-ENSRHYGFDT-KFSFNLPLLVERRDKYIQRLNNIYRQNLSKDKVDLYEGTASFL------------EGRNILIAVGNKPVFPPVKGIENTISSDEFFNIK-ESKKIGIVGSGYIAVELINVIKRLGIDSYIFARGNRILRKFDESVINVLENDMKKNNINIVTFADVVEIKKVSDKNLSIHLSDGRIYEHFDHVIYCVGRSPDTENLKLEKLNVETN-NNYIVVDENQRTSVNNIYAVGDCCMVKFYNVQLTPVAINAGRLLADRLFLKK-TRKTNYKLIPTVIFSHPPIGTIGLSEEAAIQIYGKENVKIYESKFTNLFFSVYDIEPELKEKTYLKLVCVGKDELIKGLHIIGLNADEIVQGFAVALKMNATKKDFDETIPIHPTAAEEFLTL-

General information: TITO was launched using: RESULT: Template: 1ONF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2300 -106858 -46.46 -249.67 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.83 3D Compatibility (PKB) : -46.46 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.558

(partial model without unconserved sides chains): PDB file : Tito_1ONF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1ONF-query.scw PDB file : Tito_Scwrl_1ONF.pdb: