Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLKA----IKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHRDEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPD----SAVYCGTKFAVRAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIASAVAFAIETPDRMSVSNMIIRPTTQEV 3LF2 Chain:B ((5-173)) --DLSEAVAVVTGGSSGIGLATVELLLEAGAAVAFCARDGERLRAAESALRQRFPGARLFASVCDVLDALQVRAFAEACERTLGCASILVNNAGQGRVSTFAETTDEAWSEELQLKFFSVIHPVRAFLPQLESRADAAIVCVNSLLASQPEPHMVATSAARAGVKNLVRSM----------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3LF2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 790 -56331 -71.30 -349.88 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain B : 0.71 3D Compatibility (PKB) : -71.30 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.541

(partial model without unconserved sides chains): PDB file : Tito_3LF2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3LF2-query.scw PDB file : Tito_Scwrl_3LF2.pdb: