Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKDLGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLVINHSSDQHQWFEESKKS-KESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEY-SHETGQYYLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATENPFSPFQNVSGIEEHLTDLKHVFE--EYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFDHIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTR-------PDFWAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKTREEALDIIRETTRDNSRTPMQWTSE--------------QYAGFSTH---EPWLITNPNTKTINVEQQEYEPESVLQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLA-DYELPKEADQSWTLLLSNSSSG----------EFEAKGILAPYEARLYKTNK 5M99 Chain:A ((1-506)) -----NEVKYPVVYEIFIRSLYDSDGDGVGDINGVSQKVDYLRKLGIDAVWFMPFNEAVS-YHGYDITDYYNVEKDYGTMEDLENMIQVLHENGIKVIMDLVINHTSDEHPWFKDAVENTTSSPYWDYYIMSLEDHS-GQDHW-------HWKINSKGQKVWYFGLFGYNMPDLNHDSQKVREEVKKIVDFWISKGVDGFRIDAAKHIYGWS-----------WDDGIQESAEYFEWFRDYVLSKKPDAILVGEVFSGNTYDLS------LYPIPVFNFALMYSIRN-Y-PEGQ-D---GMIEN-----NWVEESFLFLENHDLHRFFSHLQEHYKKFSESDYEFIKKRAALWYFLIFTLKGSPVIYYGGEIGTRGFKWH-------------------------------GPVYDEPVREPMQWYASGTGEGQTFWTKEVYKNAGITFGNADVDGCIYDDPYDGFSVEEQENDPKSLLNFIRFILNFRKDHDAILNGDQTIFR-DWKNLIAFYRESSNEKLLVVLNPDPVWQNSFTFEEN----MTMILEVDFENFIWNESNVSFSAGESFTVDPMKAYIFKK--

General information: TITO was launched using: RESULT: Template: 5M99.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2632 -26410 -10.03 -56.55 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -10.03 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.465

(partial model without unconserved sides chains): PDB file : Tito_5M99.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5M99-query.scw PDB file : Tito_Scwrl_5M99.pdb: