Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLENDIKKVLVSHDEITEAAKKLGAQLTKDYAG------KNPILVGILKGSIPFMAELVKHIDTHIEMDFMMVSSYHGGTASSGVINIKQDVTQDIKGRHVLFVEDIIDTGQTLKNLRDMFKEREAASVKIATLLDKPEGRVVEIEADYTCFTIPNEFVVGYGLDYKENYRNLPYIGVLKEEVYSN 4RHX Chain:D ((21-200)) ----DIKSVLLTAEQIQARIAELGEQIGNDYRELSATTGQDLLLITVLKGAVLFVTDLARAIPVPTQFEFMAVSS----------VRILKDLDRDIHGRDVLIVEDVVDSGLTLSWLSRNLTSRNPRSLRVCTLLRKPDAVHANVEIAYVGFDIPNDFVVGYGLDYDERYRDLSYIGTLDPRVY--

General information: TITO was launched using: RESULT: Template: 4RHX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 802 9719 12.12 59.26 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain D : 0.82 3D Compatibility (PKB) : 12.12 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.573

(partial model without unconserved sides chains): PDB file : Tito_4RHX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4RHX-query.scw PDB file : Tito_Scwrl_4RHX.pdb: