Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQQQVIIVGGGMVGLSLSLMLAKA-----NIAVKLLEAVKYPNYDDQNVAPYHSSFDARNTALSRRSVQIYQKLGLWDALQQHATPILQVHITEQGSFGKA-RLIA--E--QEKVESFGQVIENAWLGRVLLTQVRQQP--LIELIDGVQVTALTQDA----------------------------------------------EQVHIEAQRG---DEVLKLESKLLIAADGRDSFCRQAIGVGVDVHDYDQVAIVTTVQT--SKPHE-HVGFERFSALGPLALLPLPGEYRRSVVWPVKKGTEGEWLGEENDQHFLDALQKTYGDRAGKFEKTGKRFSYPLSQVLAHKQA-VGRIILMGNAAHTIHPVAGQGFNLCLRDADVLLRYLVNQLSASDDIGNPDNLLAYEEARLSDQQRVIKFCDTVVRGFSNQNPLLKLIRNTGLIAFDVIPGVKPLVANYAMGLKA 1PN0 Chain:A ((7-425)) SYCDVLIVGAGPAGLMAARVLSEYVRQKPDLKVRIIDKRSTK------------VYNGQADGLQCRTLESLKNLGLADKILSEANDMSTIALYNPDENGHIRRTDRIPDTLPGISRYHQVVLHQGRIERRILDSIAEISDTRIKVERPLIPEKMEIDSSKAEDPEAYPVTMTLRYMSEDESTPLQFGHKTENGLFRSNLQTQEEEDANYRLPEGKEAGEIETVHCKYVIGCDGGHSWVRRTLGFEMIGEQTDYIWGVLDAVPASNFPDIRSRCAIHSAESGSIMIIPREN-NLVRFYVQLQA-------TKFTPEVVIANAKKIFHPYTFDVQQLDWFTAYHIGQRVTEKFSKDERVFIAGDACHTHSPKAGQGMNTSMMDTYNLGWKLGLVLTG-R--AKRDILKTYEEERQPFAQALIDFDHQFSRLFSG-----------------------------------

General information: TITO was launched using: RESULT: Template: 1PN0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1802 25522 14.16 74.19 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 14.16 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.426

(partial model without unconserved sides chains): PDB file : Tito_1PN0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1PN0-query.scw PDB file : Tito_Scwrl_1PN0.pdb: