Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSQLSDINQMNMTTANTARLLITCEDKPGIVQAVSSFLYHQGANITALDQYATEAQGGRYFMRVEFELDHLQSRKDALIQTFAANVAERYGMQWRLAFVNDIKKVGILVSKVDHALLELLWRHARGSLPCEITHVISNHEDLREAVENFGILFTVIKVTKDNKAEAYAQIHEMMQ--GNDLLVLARYMQILSEDFVSKWEMKIINIHHSFLPAFVGANPYKQAYEKGVKLIGATAHYVTADLDQGPIIEQDVERVSHDYNVEQLRELGEDVERNVLARAVKWHLEDRIIVDGNKTVVF 3LOU Chain:A ((8-291)) --------------PHQFVLTLSCPSAAGQVAAVVGLLDRHRCYVDELTVFDDDL-SARFFVRCVFHATD-L-RVDALRREFE-PIAERFRMQWAIHDVAARPKVLIMVSKLEHCLADLLFRWKMGELKMDIVGIVSNHPDFAPLAAQHGLPFRHFPITADTKAQQEAQWLDVFETSGAELVILARYMQVLSPEASARLANRAINIHHSFLPGFKGAKPYHQAHARGVKLIGATAHFVTDDLDEGPIIEQVVERVDHSYRPEQLLAVGRDVECITLARAVKAFIERRVFLNGDRTVVF

General information: TITO was launched using: RESULT: Template: 3LOU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1385 -177173 -127.92 -637.31 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -127.92 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.559

(partial model without unconserved sides chains): PDB file : Tito_3LOU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3LOU-query.scw PDB file : Tito_Scwrl_3LOU.pdb: