Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPLLQIAEPGQSSAPHQHRIAIGIDLGTTHSLAATVLSGKPKVLNDVQNRRLLPSIVHYGDNTTHYGEEAKPFIIADPKNTIVSVKRFMGRSKADI--KF---QHPYELVGSEKNEMPAF--ETRAGRKTPVEISAEILKQLKDRAEDSLQNPVNGAVITVPAYFDEAQRQATRYAAQLAGLNILRLLNEPTAAAVAYGLDQESNLATDRNYVIYDLGGGTFDVSILRFSQGVFEVLATGGHTALGGDDLDRLIVKWAKKQLNIDV-----LSDEDYAVFIVAARQAKEQLSTQDSVELKLL------EATLTLDRPTFESIIQVALDKTISVCKRVLRDAKLELTDIQNVVLVGGSTRSYAVQKAVREVFAQ-EPLCTINPDEVVAIGASITANQLIGNSQ---DGSLLLDVTPLSLGLETMGGLVERLISRNTAIPVARRQEFTTYQDGQTAMLIHVVQGERDLVEHCRSLGRFVLHGIPPMTAGQARIEVTFQVDADGLLTVSAREATSGVQAHIDIKPS-YGLSEADTERLLIEGFQHAEEDKNLRHLKETKVEAERELEALEQALKVDADLLDEKQLEALNSAKESLKAQLEGSD--IQAIEQAVQQLKVHSDAFAALRMNRHIDHALKGTKLDDWSKSN 5TKY Chain:A ((11-619)) ---------------EVYDGAIGIDLGTTYSCVAVYEGTNVEIIANEQGNFTTPSFVSFTENCRLIGEAAKNQAAMNPANTIFDVKRLIGRRFDDPTVKKDMESWPFKVVDDNGNPKVEVQYLGQTHTFSPQEISAMVLTKMKEIAETKLGKKVEKAVITVPAYFNDNQRQATKDAGAIAGLNVLRIINEPTAAAIAYGLGSGK-SDKERNVLIYDLGGGAFDVSLLNIQGGVFTVKATAGDTHLGGQDFDTNLLEYCKKEFTRKTKKDLSGDARALRRLRTACERAKRTLSSGAQTTIEIDSLFDGEDFNIQITRARFEDLNAKAFAGTLEPVAQVLKDAGIEKHQVDEIVLVGGSTRIPRIQKLLSEFFDGKKLEKSINPDEAVAYGAAVQAGILSGKATSAD-DLLLLDVVPLSLGVAMEGNIFAPVVPRGQTVPTIKKRTFTTVADNQQTVQFPVYQGERVNCEDNTLLGEFTLAPIPPMKAGEPVLEVVFEVDVNGILKVTATEKTSGRSANITIANSVGKLSTDEIEKMISDAEKFKSKCEAFSKRFEAKQQLESYISRVEEIISD---------KDKIEQALSEAMAQLEIEDSTADELKKKELALKRLVTKAMA-----------------------

General information: TITO was launched using: RESULT: Template: 5TKY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 3172 69587 21.94 121.87 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : 21.94 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.517

(partial model without unconserved sides chains): PDB file : Tito_5TKY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5TKY-query.scw PDB file : Tito_Scwrl_5TKY.pdb: