Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFMSQIVDIRAREILDSRGNPTIEADVILESGVVGRACAPSGASTGSREALELRDGDKSRYLGKGVRTAVQNVNSSIHELLVGQS--VFEQKALDEKMIAFDGTENKSKLGANATLAVSLAAAHAAAAEQKLPLFQYIANLRGQTT--LTMPVPMMNILNGGAHADNTVDIQEFMIEPVGFTSFAEALRAGAEVFHSLKSVLKKQGLNTA--VGDEGGFAPNLRSNEEAITVILQAIEQTGYKAGSDIMLALDCASSEFYKNGQYILE-----GEGNKSFTSNQFADYLAGLVKQYPIISIEDGLDESDWEGWSYLTSILGDKIQLVGDDLFVTNPKILQRGIDEKVGNSILIKYNQIGTLTETLDAIYLAKANGYTTVISHRSGETEDSTIADLAVGTAAGQIKTGSLCRSDRVSKYNQLLRIEELTKAVYRGKAEFKGLK 2AL2 Chain:B ((5-417)) --------VYARSVYDSRGNPTVEVELTTEKGVF-RSIVPSGA-TGVHEALEMRDGDKSKWMGKGVLHAVKNVNDVIAPAFVKADIDVKDQKAVDDFLISLDGTANKSKLGANAILGVSLAASRAAAAEKDVPLYKHLADLSKSKTSPYVLPVPFLNVLNGG-----ALALQEFMIAPTGAKTFAEALRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAEEALDLIVDAIKAAGHDG--KVKIGLDCASSEFFKDGKYDLDFKNPNSDKSKWLTGPQLADLYHSLMKRYPIVSIEDPFAEDDWEAWSHFFKTAG--IQIVADDLTVTNPKRIATAIEKKAADALLLKVNQIGTLSESIKAAQDSFAAGWGVMVSHRSGETEDTFIADLVVGLRTGQIKTGAPARSERLAKLNQLLRIEE----------------

General information: TITO was launched using: RESULT: Template: 2AL2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2475 55311 22.35 139.67 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain B : 0.86 3D Compatibility (PKB) : 22.35 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.86 QMean score : 0.505

(partial model without unconserved sides chains): PDB file : Tito_2AL2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2AL2-query.scw PDB file : Tito_Scwrl_2AL2.pdb: