Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLTKTVKSFGLAMGLLACSLPLYAKNNVVVVATGGTIAGAGASSANSATYTAAKVPVDALINAVPQIQDLANVSGIQALQVASESITDKELLQIARQVNELVKKPTVNGVVITHGTDTLEETAFFLNLVVHTDKPIVLVGSMRPSTALSADGPLNLYSAVALAASDDAKNKGVMVLMNDSIFAARDVTKGINIHTNAFVSQ-WGALGTLVEGKPYWFRQSVKRHTNASEFNIENIKGDALPTVQIVYGSDSMLPDAYEAYAKAGAKAIIHAGTGNGSVAKYIVPTLQNLHDQNGIQIIRSSRVPQGFVLRDAEQ-PDSKYGWVAAHDLNPQKARLLAALALTKTNDAKEIQRMFWQY 5K4G Chain:B ((3-330)) ------------------------KPQVTILATGGTIAGSGESSVK-SSYSAGAVTVDKLLAAVPAINDLATIKGEQISSIGSQEMTGKVWLKLAKRVNELLAQKETEAVIITHGTDTMEETAFFLNLTVKSQKPVVLVGAMRSGSSMSADGPMNLYNAVNVAINKASTNKGVVIVMNDEIHAAREATKLNTTAVNAFASPNTGKIGTVYYGKVEYFTQSVRPHTLASEFDIS--KIEELPRVDILYAHPDDTDVLVNAALQAGAKGIIHAGMGNGNPFPLTQNALEKAA-KSGVVVARSSRVGSGSTTQE-AEVDDKKLGFVATESLNPQKARVLLMLALTKTSDREAIQKIFSTY

General information: TITO was launched using: RESULT: Template: 5K4G.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1936 -109170 -56.39 -334.88 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain B : 0.81 3D Compatibility (PKB) : -56.39 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.526

(partial model without unconserved sides chains): PDB file : Tito_5K4G.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5K4G-query.scw PDB file : Tito_Scwrl_5K4G.pdb: