Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MASYALLEQARHLQALVLDVDGILSDGFVTLTNTGDEIKSFDIRDGLGMKLAQQAGMKVIIITGRKSNIVEKRMSDLGVDLVFQGREDKGSALREACAQFNILPSDCLYMGDDWPD---LSAFAIAGMSVTVPNGHEEVRRRADLVTQAMGGRGAVREVCDMLLIAKGIYQELLEKYLAVPH 4HGO Chain:C ((2-162)) ----------KEIKLILTDIDGVWTDGGMFYDQTGNEWKKFNTSDSAGIFWAHNKGIPVGILTGEKTEIVRRRAEKLKVDYLFQGVVDKLSAAEELCNELGINLEQVAYIGDDLNDAKLLKRVGIAGVPASAPF---YIRRLSTIFLEKRGGEGVFREFVEKVL---GIN---LEDFIAV--

General information: TITO was launched using: RESULT: Template: 4HGO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 851 -68821 -80.87 -435.58 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain C : 0.79 3D Compatibility (PKB) : -80.87 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.647

(partial model without unconserved sides chains): PDB file : Tito_4HGO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4HGO-query.scw PDB file : Tito_Scwrl_4HGO.pdb: