Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSAQSKVLITGASSGIGSVYADRFAQRGYHLILVARDTNRLDKISKDLQEK-YGLQVEFIQADLSNDQDIRKIE-DVLKNDADIEILVNNAGIALNGNFLTQDRNEIEKLLTLNITAVVRLSHAMSQSLIRK-GKGAIINLGSVLGLAPEFG-STIYGASKSFIQFFSQGLHLELKDHGVHVQAVLPSATKTEIWERSGIDL-----------------SQVP--PLMDVNDLVDAALIGFDRKETITIPVLKDENQWNNFEKSRMTLLPNFSSAEVAQRYKN 4YAI Chain:A ((3-248)) -IAGTTAFITGGASGIGFGIAQRLLANGARLVLADIRQDHLDEARQFFEERQQGRNVHTIRLDVSDRAQMAEAARECEAVMGGPDILINNAGIDPSGPFKDATYQDWDYGLAINLMGPINGIMAFTPGMRARGRGGHIVNTASLAGLTPMPSFMAIYATAKAAVITLTETIRDSMAEDNIGVTVLMPGPIKSRIHESGQNRPERFRAGSGLAETEQQLAKRVVADNWMEPTEVGDMIVDAIVHNKLY------------------------------------

General information: TITO was launched using: RESULT: Template: 4YAI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1213 -32805 -27.04 -147.11 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -27.04 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.558

(partial model without unconserved sides chains): PDB file : Tito_4YAI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4YAI-query.scw PDB file : Tito_Scwrl_4YAI.pdb: