Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKKIVAIWAQDEEGLIGKENRLPWHLPAELQHFKETTLNHAILMGRVTFDGMGRRLLPKRETLILTRNPEEKIDGVATFQDVQSVLDWYQDQEKNLYIIGGKQIFQAFEPYLDEVIVTHIHARVEGDTYFPEELDLSLFETVSSKFYAKDEKNPYDFTIQYRKRKEV 3S9U Chain:A ((2-164)) HHMRVSFMVAMDENRVIGKDNNLPWRLPSELQYVKKTTMGHPLIMGRKNYEAIG-RPLPGRRNIIVTRNEGYHVEGCEVAHSVEEVFELCK-NEEEIFIFGGAQIYDLFLPYVDKLYITKIHHAFEGDTFFPE-MDMTNWKEVFVEKGLTDEKNPYTYYYHVYEKQ--

General information: TITO was launched using: RESULT: Template: 3S9U.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 780 -114631 -146.96 -703.26 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.85 3D Compatibility (PKB) : -146.96 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.85 QMean score : 0.577

(partial model without unconserved sides chains): PDB file : Tito_3S9U.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3S9U-query.scw PDB file : Tito_Scwrl_3S9U.pdb: