Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSEVLDVVIVGGGLVGGLTALLLAQGGVQPTVLDAAPVLDVEKTLSVMNPRVLALSQATIHLLKTVNVWDDL-ARQMPYT---GMQVWNLN-GYGEINFGHESVQR---PISEQALGSMVEPSVLNVAIQQKMLEQLTDYRTQVRVTCIEQGVGCWHIQLAD---G--TALKTKLLIGADGANSFVREQAFIDLDVLDYKQAAISCAIKTSKPH----HYVARQIFLPTGPLAYLPMASLEESENGYWQSIVWTLPDDYADEYSALSDREFMQLLTRESQHMLGEVLDVRSRAQFPLKARAAKQYVKAGLALIGDAAHVIHPLAGQGVNIGCLDAAVLCDALLHDLGRGVWAHEQTLMRYEHRRKGQNDAMMHSMSAIGWLESSELFPLIWARNVGLKQVEQISFLKERFMQQANGLGALQNTQYSR 2R0G Chain:A ((24-374)) -PIETDVLILGGGPVGMALALDLAHRQVGHLVVEQTDGTI-------THPRVGTIGPRSMELFRRWGVAKQIRTAGWPGDHPLDAAWVTRVGGHEVYRIPLGTADTRATPEHTPEPDAICPQHWLAPLLAEAV---GERLRTRSRLDSFEQRDDHVRATITDLRTGATRAVHARYLVACDGASSPTRKALGIDAPPRHRTQVFRNILFRAPELRSLLGERAALFFFLMLSSSLRFPLRAL---DGRGLYRLTVGVD-----------TMDSFELVRRAVAF-DTEIE-VLSDSEWHLTHRVADSFSAGRVFLTGDAAHTLSPSGGFGMNTGIGSAADLGWKLAATLRGW--AGPGLLATYEEERRPVAITSLEEA----------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2R0G.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1821 -66593 -36.57 -202.41 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -36.57 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.379

(partial model without unconserved sides chains): PDB file : Tito_2R0G.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2R0G-query.scw PDB file : Tito_Scwrl_2R0G.pdb: