Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRTLIIISHPTIEESLNQQFFKE---ASAG--LPATWHHLESSYPD--------------------------------GKIDIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERA----------AHVLKGKELGIVLTTGVAEKEYQAGGKEEYTISEFLRP-YQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQQYLSL-EGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQEMKGTSL 1GG5 Chain:A ((3-230)) RRALIVLAHSERT-SFNYAMKEAAAAALKKKGWEVVESDLYAMNFNPIISRKDITGKLKDPANFQYPAESVLAYKEGHLSPDIVAEQKKLEAADLVIFQFPLQWFGVPAILKGWFERVFIGEFAYTYAAMYDKGPFRSKKAVLSITTGGSGSMYSLQGIH-GDMNVILWPIQSGILHFCGFQVLEPQLTYSIGHTPADARIQILEGWKKRLENIWDETPLYFAPSSLFDL--------------------------------------------

General information: TITO was launched using: RESULT: Template: 1GG5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 778 -32309 -41.53 -180.50 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -41.53 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.550

(partial model without unconserved sides chains): PDB file : Tito_1GG5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1GG5-query.scw PDB file : Tito_Scwrl_1GG5.pdb: