Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKFKKMLTLAAIGLSGFGLVACGNQSAASKQSASGTIEVISRENGSGTRGAFTEITGILKKDGDKKIDNTAKTAVIQNSTEGVLSAVQGNANAIGYISLGSLTKSVKALEIDGVKASRDTVLDGEYPLQRPFNIVWSSNLSKLGQDFISFIHSKQGQQVVTDNKFIEAKTETTEYTSQHLSGKLSVVGSTSVSSLMEKLAEAYKKENPEVTIDITSNGSSAGITAVKEKTADIGMVSRELTPEE--------GKSLTHDAIALDGIAVVVNNDNKAS-QVSMAELADVFSGKLTTWDKIK 4LVQ Chain:A ((51-165)) ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------LKASGSTAQANAMTRFVNVFEQACPGQTLNYTANGSGAGISEFNGNQTDFGGSDVPLSKDEAAAAQRRCGSPAWNLPVVFGPIAVTYNLNSVSSLNLDGPTLAKIFNGSITQWNN--

General information: TITO was launched using: RESULT: Template: 4LVQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 322 -10504 -32.62 -99.09 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -32.62 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.488

(partial model without unconserved sides chains): PDB file : Tito_4LVQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4LVQ-query.scw PDB file : Tito_Scwrl_4LVQ.pdb: