Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLDSKQCEAFLAVAEVGSFDAAGEYLCITPSAVSLRVQALEKYLGQILIIR-GRPCVLTQAGQTLLQHLRHTRLMEQNLLQGLMGKSSDSEFYKIALA-SNADSLATWLLPSIQQTLFKEKIVLELKIDDQSHTHTLLETGQVNACITAEEQVMAGCLAQPLGKMRYKMLASAEFVNRWFSGGINREVLRKTPAVIFNHKDLMHSEVLLKGYGLPMQSYPYSFIPATDAFVKAIQLGLGYGMVPELQVQPLLENETLVDIMPEAQLDVPLYWHHWKRQSKQLDVLTETIVESAKQILR 3HHG Chain:A ((4-237)) --NSEELTVFVQVVESGSFSRAAEQLAMANSAVSRIVKRLEEKLGVNLLNRTTRQLSLTEEGA---QYFRRAQRILQEMAAAETEMLAVHEIPQGVLSVDSAMPMVLHLLAPLAAKFNERYPHIRLSLVSSEGYINLIER-KVDIALRAGELDDSGLRARHLFDSRFRVIASPEYLAKHGTPQSTEE-LAGHQCLGFTEPGSLNTWAVLDAQGNPYKISPH-FTASSGEILRSLCLS-GCGIV-------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3HHG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 717 -24584 -34.29 -105.97 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -34.29 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.189

(partial model without unconserved sides chains): PDB file : Tito_3HHG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3HHG-query.scw PDB file : Tito_Scwrl_3HHG.pdb: