Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPLDQLQKLSEEDIAQTIEAEQLYFQHK----PHTVYYLAVNGAKTRNGGLVRAGNDNYKANGIAIACVGDEAIYADGTTSKIISG-----------AGQACKINGVSAALVGSRLENGDEIIDTPVSLFAVRIYKDQPLPEGFLSHE 1FZ0 Chain:A ((126-251)) ------------DSAQAAEQKNGYLAQVLDEIRHTHQCAYVNYYFAKNGQDPAGHNDARRTRTIGPLWKGMKRVFSDG----FISGDAVECSLNLQLVGEACFTNPLIVAVTEWAAANGDEI--TPTVFLSIETDELRHMANGY----

General information: TITO was launched using: RESULT: Template: 1FZ0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 229 -6409 -27.98 -57.73 target 2D structure prediction score : 0.33 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -27.98 2D Compatibility (Sec. Struct. Predict.) : 0.33 1D Compatibility (Hydrophobicity) : 0.70 QMean score : -0.108

(partial model without unconserved sides chains): PDB file : Tito_1FZ0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1FZ0-query.scw PDB file : Tito_Scwrl_1FZ0.pdb: