Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNKLMVAVFAGMVPLASFSAQAATPEQECQKLQDDYNLIYASKGFCFKDQDAKEKYG--NENCHTTKPKFSDKEQQRLDAIKERQKELKCK 3WWK Chain:B ((1-32)) ---------------------------DCPSGWSSY------EGHCYKPFNEPKNWADAERFCKL--------------------------

General information: TITO was launched using: RESULT: Template: 3WWK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 42 3634 86.52 121.13 target 2D structure prediction score : 0.37 Monomeric hydrophicity matching model chain B : 0.61 3D Compatibility (PKB) : 86.52 2D Compatibility (Sec. Struct. Predict.) : 0.37 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.311

(partial model without unconserved sides chains): PDB file : Tito_3WWK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3WWK-query.scw PDB file : Tito_Scwrl_3WWK.pdb: