Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLNQHEINLKQYQNKSLSYLNSTAHSEGVEFDKFIKEIDEYPNAVVLDLGCGGGHVAYNVARHADL-VFAYDLSHEMLDTVSKAANARKI-KNIFVQQGIAEDMPFTDEQFDVIISRYSAHHWQHVPTAMKEINRVLKPNGTVIFVDIISSSFPILDTFLQAIEVIRDPSHVRNYSIKDWVHFIEDAGFELTILEKQTLKLDFDSWVQRMKTSEDQIKTLRYLQENAADVVKKYFNIQKDGSFESKVGYFVFKKLSF 5GM1 Chain:A ((67-183)) -------------------------------DYLIETLDPKAGQHLLDIGCGTGRTALKAARQRGIAVTGVAVSKEQIAAANRLAAGHGLTERLTFEVADAMRLPYEDESFDCAWAIESLCHMDRA-KALGEAWRVLKPGGDLLVLESV------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5GM1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 581 -39971 -68.80 -347.57 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -68.80 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.522

(partial model without unconserved sides chains): PDB file : Tito_5GM1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5GM1-query.scw PDB file : Tito_Scwrl_5GM1.pdb: