Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGIVKRSFNMTFAIKKIHHVAYRCKDAKETVEWYKKMLNMDFILAFAEDHVPSTKAFDPYMHLFLDAGQGNVLAFFE-------LPTQPEMGRDENTPQWVQHIAFEVE--DLNALLAAKKHLEDNGVKVLGITNHGI--FHSIYFFDPNGHRLELTYNDAHAEEKIAKITEEMKTEMLEEWSKTKRAPHHTHFLHEEELGA 4JH1 Chain:A ((2-116)) -------------LNGINHLCFSVSNLEDSIEFYEKVLEGELLV------------------------RGRKLAYFNICGVWVALNEEIHIPRNE-IYQSYTHIAFSVEQKDFESLL---QRLEENDVHILKGRERDVRDCESIYFVDPDGHKFEF----------------------------------------------

General information: TITO was launched using: RESULT: Template: 4JH1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 271 -16553 -61.08 -159.16 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -61.08 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.366

(partial model without unconserved sides chains): PDB file : Tito_4JH1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4JH1-query.scw PDB file : Tito_Scwrl_4JH1.pdb: