Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSEAYIIDAIRTPRGKGKKDGSLYEVKPITLLTTLLNELQQRHQLDTSKVDDIVLGCVTPIGDQGGDIAKTAAIAAGWNDDVAGVQINRFCASGLEAVNMAAMKVRSGWEDLVVAGGVESMSRIPMGSDGGPWALDPETNLKSSFVPQ---------------GVGADLIATLDGYSREDVDNFAVKSQQKAAAAQANGYFDQSVVPV--KDHAGVVILEKDEFIKGNTTLEGLAKLNPSFEMMGQMGFDAIALQKYPEAQKVNHVHHAGNSSGIVDGAALVLLASEKAVKEQNLKPRAKVLATALVGTDPTIMLTGPAPAARKALEKAGLTIDDIDLFEVNEAFAAVVMRFINELNVPAEKVNVNGGAIALGHPLGATGAMILGTLLDELERQNKKRGLATLCVGGGMGIATIIERV 4XL2 Chain:A ((1-392)) MKEVVIASAVRTAIGSYGK--SLKDVPAVDLGATAIKEAVKKAGIKPEDVNEVILGNVLQAG-LGQNPARQASFKAGLPVEIPAMTINKVCGSGLRTVSLAAQIIKAGDADVIIAGGMENMSRAPYLANNARWGYRMGN---AKFVDEMITDGLWDAFNDYHMGITAENIAERWNISREEQDEFALASQKKAEEAIKSGQFKDEIVPVVIKGRKGETVVDTDEHPRFGSTIEGLAKLKPAFKKDGTV--------------------TAGNASGLNDCAAVLVIMSAEKAKELGVKPLAKIVSYGSA---------GPFYATKAAIEKAGWTVDELDLIESNEAFAAQSLAVAKDLKFDMNKVNVNGGAIALGHPIGASGARILVTLVHAMQKRDAKKGLATLCIGGGQGTAILLEKC

General information: TITO was launched using: RESULT: Template: 4XL2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2279 23534 10.33 64.30 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : 10.33 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.448

(partial model without unconserved sides chains): PDB file : Tito_4XL2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4XL2-query.scw PDB file : Tito_Scwrl_4XL2.pdb: