Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMNSSTEQIISNSLSLRLKQETAAEHERMHQLMSE---AKVFSSKEKYAQFTLSQYYFQLEIEHLFEKEGV-AGLIPD-L-DIRGRSKQALADLNDL-GIQPNGQ----Q---------LQSENVQLPEALGWIYVSE-GSTLGAAFLFKEAQKHLGFS-ETFAARNLAAYPEGRAKVWKR-FVKALDEAGFDQTQQDRVVQGALDAFGYFGQALDQLDELK 2RGZ Chain:B ((30-238)) ----------MADLSELLKEGTKEAHDRAENTQFVKDFLKGNIKKELFKLATTALYFTYSALEEEMERNKDHPAFAPLYFPMELHRKEALTKDMEYFFGENWEEQVQAPKAAQKYVERIHYIGQNEPELLVAHAYTRYMGDLSGGQVLKKVAQRALKLPSTGEGTQFYLFENVDNAQQFKQLYRARMNALDLNMKTKERIVEEANKAFEYNMQIFNELD---

General information: TITO was launched using: RESULT: Template: 2RGZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 679 50481 74.35 271.40 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain B : 0.63 3D Compatibility (PKB) : 74.35 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.390

(partial model without unconserved sides chains): PDB file : Tito_2RGZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2RGZ-query.scw PDB file : Tito_Scwrl_2RGZ.pdb: