Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDDLPHSSWIPTGQGSSSMNTMTKNKLFGLADDRTDVWATPQDFFEKLDRVFKFDLDVCAL-PDNAKCERFFTPEIDGLKQEW-----TGTC-------WMNPPYGKEIIDWVAKAADTASKGHTVVALVPVRTDARWFQDYCLGREIHFIRGRLKFGGSKTNAPFGCCVVVFRPSLVDVNWEKSA 1DF0 Chain:A ((323-409)) -----------------------------------EFWMSFSDFLRHYSR-----LEICNLTPDTLTCDSYKKWKLTKMDGNWRRGSTAGGCRNYPNTFWMNPQY---------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1DF0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 45 3133 69.62 60.25 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : 69.62 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.526

(partial model without unconserved sides chains): PDB file : Tito_1DF0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1DF0-query.scw PDB file : Tito_Scwrl_1DF0.pdb: