Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKHQFKLSDPRLLSRIGYQFKQPELLQLALTHRSVSHKYNYERLEFLGDSLLGMIIANYLYHAYPHENEGRLTRMRATLVRQEALGKIATDLQLSRCLILSTGELKSGGHHRESILADTVEAIIGAIYLDSS-DLNLLKDIVLKWYTP-YLDHIEPTDQLKDPKSRLQEYLQARKKPLPVYEVVDIQGDAPHQHFKVECLVDGLSKIHGEGASRRFAEQAAAAEILKLLEQ 1RC7 Chain:A ((6-219)) -----------QLEKKLGYTFKDKSLLEKALTHVSYSKKEHYETLEFLGDALVNFFIVDLLVQYSPNKREGFLSPLKAYLISEEFFNLLAQKLELHKFIRIKRGKI------NETIIGDVFKALWAAVYIDSGRDANFTRELFYKLFKEDILSAIKEGRVKKDYKTILQEITQKRWKERPEYRLISVEGPHHKKKFIVEAKIKEYRTL-GEGKSKKEAEQRAAEELIKLLEE

General information: TITO was launched using: RESULT: Template: 1RC7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 880 -42489 -48.28 -200.42 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.82 3D Compatibility (PKB) : -48.28 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.535

(partial model without unconserved sides chains): PDB file : Tito_1RC7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1RC7-query.scw PDB file : Tito_Scwrl_1RC7.pdb: