Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNILIANDDGVFAPGIQALADALKPLGRVVVVAPESERSGFSSALTLDRPLRPIQIAE-DVWAVNGTPADCVYLSMNGLFDFEFDLVVSGINRGANLGDDVLYSGTVGAAFEGRLMKQPAIAVSLAGPDVRSYDHKDDYAQAAKWVHDFITKGLPALPPR--HIFNINIPDVP--QLKGTQITYQGRRAQSKPITSHVDPRGRQVYWIGLAGEAVTDPQRIASQIQSDFFAVANGFVSVTPIQMD---ATNYAVLEDLQASLG 4XEP Chain:A ((15-262)) MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNSLTLESSLRTFTFDNGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVIYSGTVAAAMAGRHLGFPALAVSLNG-----YQHYD----TAAAVTCALLRGLSREPLRTGRILNVNVPDLPLAQVKGIRVTRCGSRHPADKVIPQEDPRGNTLYWIGPPGD------KYDAGPDTDFAAVDEGYVSVTPLHVDLTAASAHDVVSDWLDSVG

General information: TITO was launched using: RESULT: Template: 4XEP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1364 -43756 -32.08 -182.32 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.83 3D Compatibility (PKB) : -32.08 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.464

(partial model without unconserved sides chains): PDB file : Tito_4XEP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4XEP-query.scw PDB file : Tito_Scwrl_4XEP.pdb: