Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLKKGVFYIGAVLMVGCTTLPDHSAPKEKTPPVSEKKKSESSSGVKITPYDHPEIQRKSLQVI------APQQKKPQRFNDDGSQLPAFKTLMQKTEQAY--KNQQWSEAERYALQAQRLAPQAAETYLFLAL-TANHKQQYSNAESLARRGLSFAQSQAMKKQLWLVILKAGQQRNNPKTVQEAQQALKAF 2WIN Chain:A ((134-270)) -----------LLPVGRTVMVNIENP-EGIPVKQDSLSSQNQLGVLPLSWDIPELVNMGQWKIRAYYENSPQQVFSTEFEVKEYVLPSFEVIVEPTEKFYYIYNEKGLEV---TITARFLYGKKVEGTAFVIFGIQDGEQRISLPESLKR------------------------------------------

General information: TITO was launched using: RESULT: Template: 2WIN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 302 21485 71.14 170.51 target 2D structure prediction score : 0.28 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 71.14 2D Compatibility (Sec. Struct. Predict.) : 0.28 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.060

(partial model without unconserved sides chains): PDB file : Tito_2WIN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2WIN-query.scw PDB file : Tito_Scwrl_2WIN.pdb: