Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIIMSFCVACGHKTEQKIPLGDHKVRRVCTHCGNIHYENPKVICGALALWEDK------VLLCRRAIEPRYGLWTLPAGYMELFETMEQGAARETREEAEAEI---EIEQLYCMY---NIPRIGQIYVLFKAQLKDGLFGAGEESIECRLFEEHEIPWGELAFPSVEHTLRHYFEDRKQQVFPTHLETLGTRLDHTG 2FML Chain:A ((35-156)) ------------------------------------YEKPSLTVDMVLLCYNKEADQLKVLLIQRKGHPFRNSWALPGGFVNRNESTEDSVLRETKEETGVVISQENIEQLHSFSRPDRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVHWF----------------------------------------------

General information: TITO was launched using: RESULT: Template: 2FML.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 398 5022 12.62 48.75 target 2D structure prediction score : 0.33 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 12.62 2D Compatibility (Sec. Struct. Predict.) : 0.33 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.484

(partial model without unconserved sides chains): PDB file : Tito_2FML.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2FML-query.scw PDB file : Tito_Scwrl_2FML.pdb: