Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIVQLKKDYSSDHAFFITPPAVDETKQK-----YRNNKLIKEYISVLTEVAKR-HDCEVSIFIRNFLNKWIRRIFIMG 2HSJ Chain:B ((118-181)) ----VARDYPLTEIKLLSILPVNEREEYQQAVYIRSNEKIQNWNQAYQELASAYMQVEFVPVFDCLTD----------

General information: TITO was launched using: RESULT: Template: 2HSJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 127 -2905 -22.87 -50.08 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain B : 0.65 3D Compatibility (PKB) : -22.87 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.650

(partial model without unconserved sides chains): PDB file : Tito_2HSJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2HSJ-query.scw PDB file : Tito_Scwrl_2HSJ.pdb: