Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MASLQSRLAPITNINNNAYTTTAGAPILRKPEMTSAASAVYISRKEAAVSSSHGAPTNKMMATAAVTGSSRCEEATGRRSGSKRDHETSVTTDQNPQDAAKSLPPPPKKKKVTYALPHQNMEEGHFYVVLGEDIDVSTQRFKILSLLGEGTFGKVVESWDRKRKEYCAVKIVRNVPKYTRDAKIEIQFMEKVRQADPADRFPLMKIQ-----RYFQNDSGHMCIVMPKYGPCLLDWIMK--HGPFNHRHLAQIVFQTGVALDYFHSELHLMHTDLKPENILMETSDTTV------DPATNRHLPP------DPCRVRICDLGGCCDERHSRTAIVSTRHYRSPEVILGLGWMYSTDMWSMGCIIYELYTGKLLYDTHD------NLEHLHLMEKTLGRLPSEWAARCGTEEARLLYNSAGQLRPCTDPKHLARIARARTVRDVIRDDL---------LCDLIYGLLHYDRQKRLNARQMTTHPYVLKYYPEARQAPSYPDNRAMLRPPPIM 2X7G Chain:A ((27-387)) --------------------------------------------------------------------------------------------------------------------------------------DLFNGRYHVIRKLGWGHFSTVWLCWDMQGKRFVAMKVVKSAQHYTETALDEIKLLKCVRESDPSDPNKDMVVQLIDDFKISGMNGIHVCMVFEVLGHHLLKWIIKSNYQGLPVRCVKSIIRQVLQGLDYLHSKCKIIHTDIKPENILMCVDDAYVRRMAAE-----LLVNPLDPRNADKIRVKIADLGNACWVHKHFTEDIQTRQYRSIEVLIGAGYSTPADIWSTACMAFELATGDYLFEPHSGEDYSRDEDHIAHIIELLGSIPRHFA--LSGKYSREFFNRRGELR------HITKL-KPWSLFDVLVEKYGWPHEDAAQFTDFLIPMLEMVPEKRASAGECLRHPWL--------------------------

General information: TITO was launched using: RESULT: Template: 2X7G.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1453 -62607 -43.09 -206.62 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -43.09 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.472

(partial model without unconserved sides chains): PDB file : Tito_2X7G.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2X7G-query.scw PDB file : Tito_Scwrl_2X7G.pdb: