Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSSRSYYSGSGTTAVVPHTISISTAETTPSQAPSSHTEQPSSDQQHESSLHQATASLPMSLNSTMNNKGASAAAAAAAAAPSSHGGDSHSIGAAAKASGSERANWNNPSFAAALRNSSGSINVDGYPPAQAHQQPSHPSLLSHSTDVDDEGDGAQHAAVNEPLEYCGVRLLPGVEQIDFDEGYYVGTIDENGEMAGYGKATWHSGDTYEGEWLNGMMHGKGTYTWADGDYYQGNYVRGRMEGRGEMKDATGLYTGEWADDMRQGYGRMLYAGGNVYEGEWLAGMRHGSGKLVEPAAHVTYEGEFNRNEKEGRGVQTNSDGDVYEGEFARGKPNGRGTYLWADGARYIGMFKDGVKHGDGCEWLANGDWVAGLFVDGEHVDSQSTRHKATVLTPDSSDADGGSEDGAGDGGAGGSRGSIGLSESTAAAVLAPLDAEKLRRIADQVHAPSFSMNVSAAAMRSLRSSLRKNSSSHYQDFSGPGGVTIEEASSPFLRSLAAPQMLLDDSDLEGWTPLKTIGKGSFGAVYTAL-LRNGRTVCCKVIELGTVESEEEMEKLRNEIALMRRLRHPNCVQYYGSLEDKVQNTLNIFMEYVSGGTLTSFVA-------KFKSIPLETLRQWVYQMVCGVKYLHECGIVHRDIKGDNVLVSVDGIVKLADFG-CSKAIDDVCSATHGCSTMVGTPYWMAPEVIKCEAGGYGVKSDIWSIGCTIVEMLTGKPPWPECNSMWAAVYKIANSTGLPTEIPADIDPELMDLLQRCFERNPKLRPTAADMLSHPFLAKVTEGVASPLEKSGRK 4ZJI Chain:A ((24-281)) -----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------YTRFEKIGQGASGTVYTAMDVATGQEVAIKQMNL--------KELIINEILVMRENKNPNIVNYLDSY--LVGDELWVVMEYLAGGSLTDVVTETCMDEGQIAAVCRECLQ--------ALEFLHSNQVIHRDIKSDNILLGMDGSVKLTDFGFCAQ----ITP------TMVGTPYWMAPEVVTRKA--YGPKVDIWSLGIMAIEMIEGEPPYLNENPL-RALYLIATNGTPELQNPEKLSAIFRDFLNRCLEMDVEKRGSAKELLQHQFLK-----IAKPL------

General information: TITO was launched using: RESULT: Template: 4ZJI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1157 -40687 -35.17 -170.95 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -35.17 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.464

(partial model without unconserved sides chains): PDB file : Tito_4ZJI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZJI-query.scw PDB file : Tito_Scwrl_4ZJI.pdb: