Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence QCNHHLLYNGRHWGTIRKKAGWAVRFYEEKPGQPKRLVAICKNASPVHCNYLKCTNL------AA----GFSAGTSTDVLSSGTVGSIGNDPQAQRQ 2E6C Chain:C ((68-113)) ---------------------------------------YRVRGTPADCVALGLHLFGPVDLVLSGVNLGSNLG--HEIWHSGTVAA----------

General information: TITO was launched using: RESULT: Template: 2E6C.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 40 -3119 -77.98 -86.64 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain C : 0.63 3D Compatibility (PKB) : -77.98 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.282

(partial model without unconserved sides chains): PDB file : Tito_2E6C.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2E6C-query.scw PDB file : Tito_Scwrl_2E6C.pdb: