Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQSLMQAPLLIALGLLLAAPAQAHLKKPSQLSSFSWDNCDEGKDPAVIRSLTLEPDPIIVPGNVTLSVMGSTSVPLSSPLKVDLVLEKEVAGLWIKIPCTDYIGSCTFEHFCDVLDMLIPTGEPCPEPLRTYGLPCHCPFKEGTY-SLPKSEFVVPDLELPSWLTTGNYRIESVLSSS-G-KRLGCIKIAASLKGI 2E56 Chain:A ((11-141)) ----------------------------SSDASISYTYCDKMQYP--I-SINVNPCIELKGSKGLLHIFYIPRRDLKQL-YFNLYITVN----TMNLPKRK-------EVICRGSDD----------------DYSFCRALKGETVNTT-ISFSFK----GIKFSKGKYKCVVEAISGSPEEMLFCLEFVILHQP-

General information: TITO was launched using: RESULT: Template: 2E56.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 564 -25318 -44.89 -197.79 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : -44.89 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.411

(partial model without unconserved sides chains): PDB file : Tito_2E56.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2E56-query.scw PDB file : Tito_Scwrl_2E56.pdb: