Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPLRKMKIPFLLLFFLWEAESHAASRPNIILVMADDLGIGDPGCYGNKTIRTPNIDRLASGGVKLTQHLAASPLCTPSRAAFMTGRYPVRSGMASWSRTGVFLFTASSGGLPTDEITFAKLLKDQGYSTALIGKWHLGMSCHSKTDFCHHPLHHGFNYFYG----------------ISLTNLRDCKPG--EGSVFTTGFKRLVFLPLQIVGVTLLTLAALNCLGLLHVPLGVFFSLLFLAALILTLFLGFLHYFRPLNCFMMRNYEIIQQPMSYDNLTQRLTVEAAQFIQRNTETPFLLVLSYLHVHTALFSSKDFAGKSQHGVYGDAVEEMDWSVGQILNLLDELRLANDTLIYFTSDQGAHVEEVSSKGEIHGGSNGIYKGGKANNWEGGIRVPGILRWPRVIQAGQKIDEPTSNMDIFPTVAKLAGAPLPEDRIIDGRDLMPLLEGKSQRSDHEFLFHYCNAYLNAVRWHPQNSTSIWKAFFFTPNFNPVGSNGCFATHVCFCFGSY-----------------------VTHHDPPL----LFDISKDPRERNPLTPASEPRFYEILKVMQEAADRHTQTLP-----EVPDQFSWNNFLWKPWLQLCCPSTGLSCQCDREKQDKRLSR 1FSU Chain:A ((4-492)) --------------------------PHLVFLLADDLGWNDVGFHGSR-IRTPHLDALAAGGVLLDNYYT-QPL-TPSRSQLLTGRYQIRTGLQH-----QIIWPCQPSCVPLDEKLLPQLLKEAGYTTHMVGKWHLGM--YRKECL---PTRRGFDTYFGYLLGSEDYYSHERCTLIDALNVTRCALDFRDGEEVATGYKNM-------YSTNIFTKRAI-ALITNHPPEKPLFLYLALQSV----------------------HEPLQVPEEY--------LKPYDFIQ---------------------------DKNRHH-YAGMVSLMDEAVGNVTAALKSSGLWNNTVFIFSTDNGGQT--------LAGGNNWPLRGRKWSLWEGGVRGVGFVASPLLKQKGVKNRELIHISDWLPTLVKLARGHTNGTKPLDGFDVWKTISEGSPSPRIELLHNIDPNFVDSSPC------------------SAFNT----SVHAAIRHGNWKLLTGYPGCGYWFPPPSQYNVSEIPSSDPPTKTLWLFDIDRDPEERHDL---SREYPHIVTKLLSRLQFYHKHSVPVYFPAQDPRCDPKATGVWGPWM------------------------

General information: TITO was launched using: RESULT: Template: 1FSU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2671 -35354 -13.24 -83.98 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -13.24 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.411

(partial model without unconserved sides chains): PDB file : Tito_1FSU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1FSU-query.scw PDB file : Tito_Scwrl_1FSU.pdb: