Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTEQMTLRGTLKGHNGWVTQIATTPQFPDMILSASRDKTIIMWKLTRDETNYGIPQRALRGHSHFVSDVVISSDGQFALSGSWDGTLRLWDLTTGTTTRRFVGHTKDVLSVAFSSDNRQIVSGSRDKTIKLWN-TLGVCKYTVQDESHSEWVSCVRFSPNSSNPIIVSCGWDKLVKVWNLANCKLKTNHIGHTGYLNTVTVSPDGSLCASGGKDGQAMLWDLNEGKHLYTL--DGGDIINALCFSPN-RYWLCAATGPSIKIWDL-EGKIIVDELKQEVISTSSKAEPPQCTSLAWSADGQT-LFAGYTDNLVRVWQVTIGTR 5M25 Chain:A ((133-388)) -------------------------------------------------PNYAL-KFTLAGHTKAVSSVKFSPNGEWLASSSADKLIKIWGAYDGKFEKTISGHKLGISDVAWSSDSNLLVSASDDKTLKIWDVSSGKCLKTLK--GHSNYVFCCNFNPQSN--LIVSGSFDESVRIWDVKTGKCLKTLPAHSDPVSAVHFNRDGSLIVSSSYDGLCRIWDTASGQCLKTLIDDDNPPVSFVKFSPNGKYILAATLDNTLKLWDYSKGKCLK-------TYTGHKNEK-YCIFANFSVTGGKWIVSGSEDNLVYIWNL-----

General information: TITO was launched using: RESULT: Template: 5M25.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1435 -25113 -17.50 -100.45 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -17.50 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.487

(partial model without unconserved sides chains): PDB file : Tito_5M25.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5M25-query.scw PDB file : Tito_Scwrl_5M25.pdb: