Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEVPPPAPRSFLCRALCLFPRVFAAEAVTADSEVLEERQKRLPYVPEPYYPESGWDRLRELFGKDEQQRISKDLANICKTAATAGIIGWVYGGIPAFIHAKQQYIEQSQAEIYHNRFDAVQSAHRAATRGFIRYGWRWGWRTAVFVTIFNTVNTSLNVYRNK-DALSHFVIAGAVTGSLFRINVGL-RGLVAGGIIGALLGTPVGGLLMAFQKYSGETVQERKQKDRKALHELKLEEWKGRLQVTEHLPEKIESSLQEDEPENDAKKIEALLNLPRNPSVIDKQDKD 5XTD Chain:V ((12-113)) ----------------------------------------------------------------------IPDGTDCHRKAYSTTSIASVAGLTAAAYRVTLN--PPG---T--------------FLEG-VAKVGQYTFTAAAVGAVFGLTTCISAHVREKPDDPLNYFLGGCAGGLTLGARTHNYGIGAA-----------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5XTD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain V - contact count / total energy / energy per contact / energy per residue : 310 -16122 -52.00 -161.22 target 2D structure prediction score : 0.27 Monomeric hydrophicity matching model chain V : 0.62 3D Compatibility (PKB) : -52.00 2D Compatibility (Sec. Struct. Predict.) : 0.27 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.156

(partial model without unconserved sides chains): PDB file : Tito_5XTD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5XTD-query.scw PDB file : Tito_Scwrl_5XTD.pdb: