Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIHFSINKNLFLQALNITKRAISSKNAIPILSTVKIDVTNEGVTLIGSNGQISIENFISQKNEDAGLLITSLGSILLEASFFINVVSSLP-DVTLDFKEIEQNQIVLTSGKSEITLKGKDSEQYPRIQEISASTPLILETKLLKKIINETAFAASTQESRPILTGVHFVLSQHKELKTVATDSHRLSQKKLTLEKNSDD-FDVVIPSRSLREFSAVFTDDIETVEIFFANNQILFRSENISFYTRLLEGNYPDTDRLIPTDFNTTITFNVVNLRQSMERARLLSSATQNGTVKLEIKDGVVSAHVHSPEVGKVNEEIDTDQVTGEDLTISFNPTYLIDSLKALNSEKVTISFISAVRPFTLVPADTDEDFMQLITPVRTN 4TR8 Chain:A ((17-382)) -MHFTIQREALLKPLQLVAGVVE---TLPVLSNVLLVVEGQQLSLTGTDLEVELVGRVVL--EDA----AEPGEITVPARKLMDICKSLPNDVLIDIR-VEEQKLLVKAGRSRFTLSTLPANDFPTVEEGPGSLNFSIAQSKLRRLIDRTSFAMAQQDVRYYLNGMLLEVN-GGTLRSVATDGHRLAMCSLDAQIPSQDRHQVIVPRKGILELARLLTEQDGEVGIVLGQHHIRATTGEFTFTSKLVDGKFPDYERVLPRGGDKLVVGDRQQLREAFSRTAILSNEKYRG-IRLQLSNGLLKIQANNPEQ-EEAEEEVQVEYNGGNLEIGFNVSYLLDVLGVIGTEQVRF-ILSDSNSSALVHEADNDDSAYVVMPMR--

General information: TITO was launched using: RESULT: Template: 4TR8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1982 -21801 -11.00 -60.39 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : -11.00 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.504

(partial model without unconserved sides chains): PDB file : Tito_4TR8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4TR8-query.scw PDB file : Tito_Scwrl_4TR8.pdb: