Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIKELYEEVQGTVYKCRNEYYLHLWELSDWDQEGMLCLHELISREEGLVDDIPRLRKYFKTKFRNRILDYIRKQESQKRRYDKEPYEEVGEISHRISEGGLWLDDYYLFHETLRDYRNKQSKEKQEELERVLSNERFRGRQRVLRDLRIVFKEFTIRTH 4D7Q Chain:A ((10-106)) --------------------------------------------------EIEKAQREIIEAFNAKPKNGINKIKEICEQYKISPNEEIAEFFHQQRKN--------LDLEAVGDYLSSPEAENQQVLKAFTSQMNFNG-QSFVEGLRTFLKTFKL---

General information: TITO was launched using: RESULT: Template: 4D7Q.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 295 -11003 -37.30 -113.43 target 2D structure prediction score : 0.85 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -37.30 2D Compatibility (Sec. Struct. Predict.) : 0.85 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.397

(partial model without unconserved sides chains): PDB file : Tito_4D7Q.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4D7Q-query.scw PDB file : Tito_Scwrl_4D7Q.pdb: