Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKADTIFKENIERILKEGVFSEQARPKYKDGTVANSKYVTGAFSEYDLSKGEFPITTLRPIAIKSAIKEVLWIYQDQSNSLEVLNDKYNVHYWNDWEVGDTGTIGERYG-------AVVKKH-DIINKLLKQLETNPWNRRNIISLWDYQAFEETDGLLPCAFQTMFDVRRVDGEIYLDATLTQRSNDMLVAHHINAMQYVALQMMIAKHFGWKVGKFFYFINNLHIYDNQFEQAQELLRREPSNCQPRLVLNVPDGTNFFDIKAEDFELVDYDPVKPQLKFDLAI 4FQS Chain:A ((13-263)) ----------------------ETGTPK-SDRTGTGTRSLFGQQMRYDLSAG-FPLLTTKKVHFKSVAYELLWFLRGDSN-IGWLHE-HGVTIWDEW-ASDTGELGPIYGVQWRSWPAPSGEHIDQISAALDLLRTDPDSRRIIVSAWNVGEIERM-ALPPC--HAFFQFYVADGR--LSCQLYQRSADLFLGVPFNIASYALLTHMMAAQAGLSVGEFIWTGGDCHIYDNHVEQVRLQLSREP-RPYPKLLLA--DRDSIFEYTYEDIVVKNYDP-HPAIKAPVAV

General information: TITO was launched using: RESULT: Template: 4FQS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1153 -29130 -25.26 -119.87 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -25.26 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.526

(partial model without unconserved sides chains): PDB file : Tito_4FQS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4FQS-query.scw PDB file : Tito_Scwrl_4FQS.pdb: