Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDTPDENGYVADDYRITYLEAHIKAMRDAIYKDGVDLLGYTTWGCIDSVSAGTGEMNKRYGFIYVDRDNVGNGALKRSKKKSFYWYKDVIDSNGVSIE 4ZE4 Chain:A ((391-485)) FDTLEPGDIVNDDYRIDYLRRHVQEIQRAI-TDGVDVLGYCAWSFTDLLSWLNG-YQKRYGFVYVNRDDESEKDLRRIKKKSFYWYQRVIETNGAEL-

General information: TITO was launched using: RESULT: Template: 4ZE4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 284 -9714 -34.20 -102.25 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.88 3D Compatibility (PKB) : -34.20 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.88 QMean score : 0.352

(partial model without unconserved sides chains): PDB file : Tito_4ZE4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZE4-query.scw PDB file : Tito_Scwrl_4ZE4.pdb: