Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKRVTIIDVKDYVGQEVTIGAWVANKSGKGKIAFLQLRDGTAFFQGVAFKPNFVEKFGEEVGLEKFDVIKRLSQETSVYVTGIVKEDERSKFGYELDITDIEVIGESQDYPITPKEHG-----TDFLMDNRHLWLRSRKQVAVLQIRNAIIYVTYEFFDKNGFMKFDSPILSGNAAEDSTELFETDYFGTPAYLSQSGQLYLEAGAMALGRVFDFGPVFRAEKSKTRRHLTEFWMMDAEYSYLTHDESLDLQEAYVKALLQGVLDRAPQALETLERDTELLKRYIAEPFKRITYDQAIDLLQEHENDEDADYEHLEHGDDFGSPHETWISNHFGVPT-FVMNYPAAIKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREEDYDALVAKMDELGMDRTEYEFYLDLRKYGTVPHGGFGIGIERMVTFAAGTKHIREAIPFPRMLHRIKP 1WYD Chain:A ((13-429)) -----------EYDGKEVIWAGWVHLLRDLGGKKFIILRDKTGLGQVVVDKNS-----------SAFGISQELTQESVIQVRGIVKADKRAPRGIELHAEEITLLSKAK-APLPLDVSGKVKADIDTRLRERVLDLRRQEMQAVIKIQSLALKAFRETLYKEGFIEIFTPKIIASATEGGAQLFPVIYFGKEAFLAQSPQLYKELMAGVVERVFEVAPAWRAEESDTPFHLAEFISMDVEMAFADYNDVMQLLEKILHNIVKTIKEEGKEELKILNYEPPEVKI----PIKRLKYTEAIEILRSKGYN-------IKFGDDIGTPELRILNEELKEDLYFIVDWPSDARPFYTKSKSE-PELSESFDLIY--KFLEIVSGSTRNHKREVLEEALKKKGLKPESFEFFLKWFDYGMPPHAGFGMGLARLMVMLTGIQSVKEIVPFPRDKKRLTP

General information: TITO was launched using: RESULT: Template: 1WYD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2025 4708 2.32 11.48 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : 2.32 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.431

(partial model without unconserved sides chains): PDB file : Tito_1WYD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1WYD-query.scw PDB file : Tito_Scwrl_1WYD.pdb: