Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSQKMNHLDVGEFVLLLPEHLRSEE----EHYKSVFEDDLTSRISSQDERQQMTATVGYLESGQDRFVYNTTPISYQQFLKDPIIIVITPQSTGPQSILFWIDAVQNYVLFNQLSDAQELIQRQGIENWVSEMQTGYHNYITLL-DNIQRERWVMLAGAVLGIATSILLFNTMNRLYFEEFRRAIFIKRIAGLRFLEIHRTYLFAQLGVFLLGFVASVFLQVEI--GVAFLVLLLFTGLSLLQLHVQMQKENKMSMLVLKGG 4WRT Chain:C ((9-258)) -GMTLAKIELLKQLLRDNEAKTVLKQTTVDQYNIIRKFNTSRIEKNPSLRMKWAMCSNFPLALTKGDMANRIPLEYKG-----IQLKTNAEDIGTKGQMCSIAAVTWWNTYGPIGD-TEGFERVYESFFLRKMRLDNATWGRITFGPVERVRKRVLLNPLTKEMPPDEASNVIMEILF---PKEAGIPRESTWIHRELIKEKREKLKGTMITPIVLAYMLERELVARRRFLPVAGATSAEFIEMLHCLQGEN--WRQIYHPG

General information: TITO was launched using: RESULT: Template: 4WRT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 666 -31386 -47.13 -129.16 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain C : 0.63 3D Compatibility (PKB) : -47.13 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.250

(partial model without unconserved sides chains): PDB file : Tito_4WRT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4WRT-query.scw PDB file : Tito_Scwrl_4WRT.pdb: