Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLYIWMPETNGVWHWSNGENWLQAASLDQLIQDLQIHQGKEAAVFFPSRHAQMLQQTMAKFHYKQLGADGVKYLLEEFVTLPIDHMKVVHHFHADQLTVLAVAQGMVETWQHSLALLPTKLVALLPDFLVLPEPQAQQVILCNIGHQLLVRENKWLGNSIDDLGLFLEFQSAETHYQYSGLTAEQLESLEAASSAEQRSEFVYQFQPLDKTKQHPFNVLPKSKGQERTFSNYWKACAAVVLAIIVVQFSYDLLRWVKLKKVADQTAEQAIEQYKYWFGSSSGRVTEQNVRSNFKSRLEVSQQGDTQALSLLSRVGPILMQ-RQILAQQLNYDA--SILTMALKTKSADDLQALTQQLNQQGFKAELGNVQADGSGAIGVVKIQ 2W7V Chain:A ((7-85)) ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DVAMLSWLAALPATLGQVKDLEITSFKYDGQRGEVRIHARSSDFQPFEQARVKL-AEKFNVEQGQLNRS-NVVMGSFVLK

General information: TITO was launched using: RESULT: Template: 2W7V.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 278 25426 91.46 339.01 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.55 3D Compatibility (PKB) : 91.46 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.55 QMean score : 0.571

(partial model without unconserved sides chains): PDB file : Tito_2W7V.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2W7V-query.scw PDB file : Tito_Scwrl_2W7V.pdb: